What are the key steps for preserving RNA-containing specimens during transport?

Prepare for the CAMLPR Specimen Collection Competency Test. Practice with diverse question sets including multiple-choice and flashcards. Each question includes hints and explanations to help you become proficient before the exam.

RNA is highly unstable and quickly degraded by RNases that are everywhere. To keep RNA-containing specimens intact during transport, you want to prevent RNase contamination from the start by using RNase-free tubes and materials. Then stabilize the RNA with an appropriate medium designed to preserve RNA integrity so degradation doesn’t start during transit. Keeping the sample cold is essential—on ice or at 2–8°C—to slow any residual RNase activity. Finally, minimize freeze–thaw cycles, because repeatedly freezing and thawing fragments RNA and undermines its integrity. Room temperature accelerates degradation, and storing in metal containers or exposing the specimen to air doesn’t provide RNA protection or prevent contamination. Put together, these steps maintain RNA quality from collection through analysis.